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wet transfer western blot apparatus  (Thermo Fisher)


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    Structured Review

    Thermo Fisher wet transfer western blot apparatus
    Wet Transfer Western Blot Apparatus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wet+transfer+western+blot+apparatus/pm39010179-149-19-24
    Average 90 stars, based on 1 article reviews
    wet transfer western blot apparatus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Bisphenols A and F, but not S, induce apoptosis in bovine granulosa cells via the intrinsic mitochondrial pathway.
    Article Snippet: 20 mg of protein for each sample were dissolved in equal volumes of 3x Reducing Buffer plus 5% b- mercaptoethanol (Sigma Aldrich), denatured at 90°C and loaded onto a 15% polyacrylamide gel in the XCell SureLock Mini-Cell Electrophoresis System (Invitrogen). .. Gels were run at 125 V for 2 hours and proteins were transferred onto a nitrocellulose membrane (Bio-Rad) for 1 hour at 20V using the InvitrogenWet Transfer Western blot apparatus (Invitrogen) filled with 1x Towbins buffer. .. Membranes were washed in 1x Tris Buffered Saline and 0.1% Tween (TBST) (FisherBrand) and then blocked for 1 hour in the 5% skim milk or 5% BSA in TBST for BAX (Proteintech; 50599-2-IG) and BCL-2 (Proteintech; 12789-1- AP), respectively.

    Article Title: Effects of Delta-9 Tetrahydrocannabinol (THC) on Oocyte Competence and Early Embryonic Development
    Article Snippet: 8% polyacrylamide gels were prepared using Bio-Rad standard gel recipes. .. Proteins samples were loaded onto the gel in an Invitrogen wet transfer western blot apparatus for 2 h at 125V in running buffer, followed by transfer to a nitrocellulose membrane. .. The CX43 primary antibody (Sigma-Aldrich, Cat# C6219) was used at a 1:8,000 dilution overnight and the secondary antibody, Anti- Rabbit IgG HRP-linked antibody (Cell Signaling Technology, Cat# 7074) was incubated at a dilution of 1:5,000 for 1 h. The blot was washed in TBST three times and incubated with UltraScence Western Substrate (FroggaBio) for chemiluminescence for 5 min.

    Western Blot:

    Article Title: Bisphenols A and F, but not S, induce apoptosis in bovine granulosa cells via the intrinsic mitochondrial pathway.
    Article Snippet: 20 mg of protein for each sample were dissolved in equal volumes of 3x Reducing Buffer plus 5% b- mercaptoethanol (Sigma Aldrich), denatured at 90°C and loaded onto a 15% polyacrylamide gel in the XCell SureLock Mini-Cell Electrophoresis System (Invitrogen). .. Gels were run at 125 V for 2 hours and proteins were transferred onto a nitrocellulose membrane (Bio-Rad) for 1 hour at 20V using the InvitrogenWet Transfer Western blot apparatus (Invitrogen) filled with 1x Towbins buffer. .. Membranes were washed in 1x Tris Buffered Saline and 0.1% Tween (TBST) (FisherBrand) and then blocked for 1 hour in the 5% skim milk or 5% BSA in TBST for BAX (Proteintech; 50599-2-IG) and BCL-2 (Proteintech; 12789-1- AP), respectively.

    Article Title: Effects of Delta-9 Tetrahydrocannabinol (THC) on Oocyte Competence and Early Embryonic Development
    Article Snippet: 8% polyacrylamide gels were prepared using Bio-Rad standard gel recipes. .. Proteins samples were loaded onto the gel in an Invitrogen wet transfer western blot apparatus for 2 h at 125V in running buffer, followed by transfer to a nitrocellulose membrane. .. The CX43 primary antibody (Sigma-Aldrich, Cat# C6219) was used at a 1:8,000 dilution overnight and the secondary antibody, Anti- Rabbit IgG HRP-linked antibody (Cell Signaling Technology, Cat# 7074) was incubated at a dilution of 1:5,000 for 1 h. The blot was washed in TBST three times and incubated with UltraScence Western Substrate (FroggaBio) for chemiluminescence for 5 min.

    Article Title: DNA methylation, but not microRNA expression, is affected by in vitro THC exposure in bovine granulosa cells.
    Article Snippet: Samples were denatured at 90 °C and loaded onto a 15% polyacrylamide gel in the XCell SureLock Mini-Cell Electrophoresis System (Invitrogen; Burlington, ON, Canada) containing 5X Tris-Glycine Buffer. .. Gels were run for 2 h at 125 V and transferred onto nitrocellulose membranes (Bio-Rad, 1,620,115) in an Invitrogen wet transfer western blot apparatus (Invitrogen; Burlington, ON, Canada) containing 1X Towbins Buffer, for 2 h at 40 V. Membranes were blocked for 1 h in 5% skim milk in TBST. .. DNMT1 primary antibody (1:5000 in 5% skim milk in TBST) was incubated overnight at 4 °C and the anti-rabbit IgG HRPlinked secondary antibody (Cell Signalling Technology, Whitby, ON, Canada; 70,735) (1:2000 in 5% skim milk in TBST) was incubated for 1 h at room temperature.

    Article Title: BPA and BPS affect the expression of anti-Mullerian hormone (AMH) and its receptor during bovine oocyte maturation and early embryo development
    Article Snippet: .. Samples were run on the 12% gel in an Invitrogen wet transfer western blot apparatus (Invitrogen, Burlington, ON) at 125 V for 2 h. The gel was then placed in a transfer chamber full of cold 1X transfer buffer of Tris, Glycine and water and was run at 25 V for 3 h in order to transfer the protein onto nitrocellulose paper (Biorad 1,620,115). .. Nitrocellulose blots were washed 2X 10 min in Tris buffered saline pH 7.6 with 0.1% Tween 20 (Thermo Fisher Scientific, Whitby, Canada; BP337) (TBST), ponceau stained to ensure protein transfer, blocked for 1 h in 5% skim milk in TBST and incubated with each primary antibody of interest at 4 °C overnight: AMH at 1:500 (Abcam, Cambridge, United States; ab229212) and AMHRII 1:2000 (Abcam; ab197148) dilutions, respectively.



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